Supplementary data from: Xenomonitoring reveals mosquito-host feeding patterns in Serbia

Many mosquito species act as vectors for zoonotic pathogens, transmitting them between natural amplifying hosts and dead-end hosts. Determining the relationship between mosquitoes and their hosts is crucial for understanding the lifecycle dynamics of such pathogens. The aim of this research was to assess the host-feeding patterns of mosquitoes inhabiting natural and semi-natural environments of Vojvodina Province, Serbia, using the invertebrate-derived DNA (iDNA) from blood-fed female mosquitoes. Mosquito species (Diptera: Culicidae) belonging to four genera were identified, based upon morphological characteristics. A segment of the 16S rRNA gene was amplified and sequenced, allowing for ten different vertebrate host species to be identified. Culex specimens collected in this study primarily fed on birds, while mammal species constituted about a third of their bloodmeals. Species of Anopheles and Aedes overwhelmingly took bloodmeals from a single host species, the red deer, but also fed..., , ## Supplementary Table 1:

### Information on the sampled mosquitoes and their detected
host species.

Adult mosquitoes were sampled from resting sites using a handheld, battery-powered aspirator. Mosquitoes were aspirated in five different localities: Plavna, Platičevo, Karakuša, Budžak, and Kovilj, in the mornings (06:00 AM – 11:00 AM)
of mid-June and mid-July 2024.

Variables:

* Sample number
* Collection date: day, month, year
* Location: locality
* Mosquito species: What species of mosquito was sampled
* Host species: Detected host species
* Per. Ident.: (Percentage identity) stands for the
percentage of nucleotides that are the same between the examined sequence and sequence
suggested by NCBI’s BLAST function.

Data are available as csv (SupplementaryTable1.csv) and .ods (SupplementaryTable1.ods) formats.
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