Single cell transcriptome of cultured skin and airways progenitor and stem cells

Purpose: The goal of this study is to characterise expanding epithelial progenitor and stem cells in culture from different organs and compare them to thymic ones Methods: FACS-isolation protocol to separate epithelial cells from mouse feeder layer cells then loaded into the 10x Genomics Chromium Platform, and sequenced using Illumina HiSeq 4000. Conclusions: Our study dissected in vitro properties of airways and skin progenitor and stem cells and showed they differ from thymic ones Overall design: 2 FACS sorted epithelial populations were used for single-cell RNA-sequencing (10xGenomics Chromium Platform), and sequenced using Illumina HiSeq 4000.