Supplementary Material for: A Novel Role of Semaphorin 3C in Modulating Systemic and Renal Hemodynamics

<b><i>Background:</i></b> Alterations of renal hemodynamics play an essential role in renal homeostasis and kidney diseases. Recent data indicated that semaphorin 3C (SEMA3C), a secreted glycoprotein involved in vessel development, can modulate renal vascular permeability in acute kidney injury, but whether and how it might impact systemic and renal hemodynamics is unknown. <b><i>Objectives:</i></b> The objective of the study was to explore the effect of SEMA3C on systemic and renal hemodynamics. <b><i>Methods:</i></b> SEMA3C recombinant protein was administered intravenously in two-month-old wild-type mice, and the variations of mean arterial pressure, heart rate, renal blood flow, and renal vascular resistance were measured and analyzed. <b><i>Results:</i></b> Acute administration of SEMA3C induced (i) systemic hemodynamic changes, including mean arterial pressure decrease and heart rate augmentation; (ii) renal hemodynamic changes, including reduced vascular resistance and elevated renal blood flow. Continuous perfusion of SEMA3C had no significant effect on systemic or renal hemodynamics. <b><i>Conclusion:</i></b> SEMA3C is a potent vasodilator affecting both systemic and renal hemodynamics in mice.