Supplementary Files For The Manuscript "Assembly Of Long Error-Prone Reads Using Repeat Graphs"

Supplementary files for the manuscript "Assembly of Long Error-Prone Reads Using Repeat Graphs" Contents<br> --------- * `human_assemblues` - Flye assemblies of the human ONT sequencing data + QUAST benchmarking<br> of Flye, Canu and MaSuRCA assemblies. Scripts for assembly graph analysis are also included. * `nctc_assemblis` - Flye assemblies of the NCTC 21 bacterial dataset. * `yeast_assemblies` - working directories Flye, Canu, Falcon, Hinge and Miniasm assemblies of <br> yeast PB and ONT datasets + final assemblies + quast report. Some large files <br> (such as read alignments) were deleted. * `worm_assemblies` - working directories Flye, Canu, Falcon, Hinge and Miniasm assemblies of <br> the c. elegans dataset + final assemblies + quast report. Some large files <br> (such as read alignments) were deleted. `tandem_misassemblies` directory contain<br> the detailed analysis of nine tandem misassemblies. We recommend "gepard" dot-plotter for visualization. * `metagenome_assemblies` - Flye and Canu assemblies of a PacBio mock metagenome dataset.<br> In addition to metagenome assemblies, each bacteria was reassembled separately to<br> estimate the rate of divergence between the target genomes and the available references. * `simulated_data` - two assemblies of the simulated data illustrating Figure 1 (from Appendix I),<br> as well as simulated unbridged repeats benchmark. <br> Software versions and parameters<br> -------------------------------- * Flye - 2.3.5 (commit 20afeda)<br> * Canu - 1.7.1 (commit dfa60b8)<br> * Falcon - 0.3.0 (FALCON-Integrate commit 7498ef9)<br> * HINGE - 0.5.0 (commit 79fdf66)<br> * Miniasm - 0.2-r168-dirty (commit 40ec280) / Minimap2 2.8-r711-dirty (commit 8fc5f8d)<br> * Quast - 5.0.0 (commit de6973bb) Flye and Canu were run with the default parameters. The config files / scripts for<br> Falcon, HINGE and Miniasm could be found in the 'asm_config' archive folder. The HUMAN (but not the HUMAN+) assembly was generated with the earlier <br> Flye version 2.3.2 (released on Feb 20 2018) to provide a fair comparison <br> with the Canu and MaSuRCA assemblies (which were not updated since the release of Flye 2.3.2).<br> We note that the HUMAN assembly using the latest Flye version 2.3.5 has <br> NGA50 = 7.3 Mb and improves over the Flye 2.3.2 assembly (NGA50 = 6.3Mb). <br> HUMAN+ was assembled using the latest Flye and Canu versions (as of September 2018). The code for unbridged repeat resolution is currently available <br> in a separate 'flye-trestle' branch (commit 6100d32)