Positive effects of cashew gum and nanoparticles on cooled stallion semen.

The stallion sperm is widely susceptible to cold shock during storage at low temperatures. Thus, this study added CG or nanoparticles (NP) containing CG to the extender before cooling due to verified if it was possible to improve sperm quality in stallion semen. We worked with 6 treatment groups (control, a-tocopherol TOC, CG1, CG0.5, NP1 and NP0.5), stored in cryotubes at 4°C, and analyzed on the day, after 24h and 48h. The datas show the results obtained by CASA (computer-assisted sperm analysis) and Flow cytometry assays.