Identification and integrative analysis of microRNAs and mRNAs involved in proliferation and invasion in mechanical pressure-treated human hepatocellular carcinoma cell lines (mRNA)

The pressure changes in hepatic microenvironment may be involved in the regulation of proliferation and invasion of hepatocellular carcinoma, but the mechanism is unknown. This project simulates the mechanical microenvironmental conditions of liver cirrhosis, exerts the static water pressure on human hepatoma cell lines，and observes the changes of proliferation and invasion of hepatoma cells under different pressure loading conditions. Then screens the differential expression mechanic-responsive mRNAs under the optimal loading condition and carry out biological verification. Bioinformatics analysis was used to explore the related pathways. This study provides a theoretical basis for the prevention and treatment of liver cancer and provides a direction for finding effective drugs for clinical treatment of liver cancer. 4 samples are analysised, of which 2 are of control group which cultured without pressure, and 2 are of case group which cultured under pressure (15mmHg, 24h).