Data underlying the publication: Interfacial protein adsorption behavior can be connected across a wide range of timescales using the microfluidic EDGE tensiometer

# IntroductionIn the current study, we expand the use of our EDGE (Edge-based Droplet GEneration) device, and validate the findings with ADT (automated drop tensiometer) results at complementary timescales.Furthermore, we explore if the EDGE tensiometer could distinguish effects occurring at high whey protein concentrations (2.5 – 10 wt%).In this dataset, you can find an excel file with an overview of all dataset named "EDGEvsADT_WPI_20240516_v09_final.xlxs" (in the Analyzed_Data folder).You can also find the raw data in the Raw_Data folder with .txt files.Finally, in the Plotted_Data_Scripts folder you can find all processed data from the excel file in .txt extension together with a Matlab code to plot the data.<br># AbstractHypothesisOur hypothesis is that dynamic interfacial tension values as measured by the partitioned-Edge-based Droplet GEneration (EDGE) tensiometry can be connected to those obtained with classical techniques, such as the automated drop tensiometer (ADT), expanding the range of timescales towards very short ones.<br>ExperimentsOil-water and air-water interfaces are studied, with whey protein isolate solutions (WPI, 2.5 – 10 wt%) as the continuous phase.The dispersed phase consists of pure n-hexadecane or air. The EDGE tensiometer and ADT are used to measure the interfacial (surface) tension at various timescales.A comparative assessment is carried out to identify differences between protein concentrations as well as between oil-water and air-water interfaces.<br>FindingsThe EDGE tensiometer can measure at timescales down to a few milliseconds and up to around 10 seconds, while the ADT provides dynamic interfacial tension values after at least one second from droplet injection and typically is used to also cover hours.The interfacial tension values measured with both techniques exhibit overlap, implying that the techniques provide consistent and complementary information.Unlike the ADT, the EDGE tensiometer distinguishes differences in protein adsorption dynamics at protein concentrations as high as 10 wt% (which is the highest concentration tested) at both oil-water and air-water interfaces.<br># Measurements and data collectionMeasurements and data collection were done according to what is described in the paperWe used two main setups and equipment, namely ADT (Automated Drop Tensiometer) and EDGE (partitioned-Edge-based Droplet GEneration) for data collection

# 引言
在本研究中，我们拓展了EDGE（基于微流控液滴生成的Edge装置，Edge-based Droplet GEneration）的应用，并通过自动化滴形张力仪（automated drop tensiometer, ADT）在互补时间尺度上的实验结果验证了相关发现。此外，我们探究了EDGE张力仪能否区分高乳清蛋白浓度（2.5–10 wt%）下产生的效应。
本数据集包含分析数据文件夹（Analyzed_Data）中的总览文件"EDGEvsADT_WPI_20240516_v09_final.xlxs"。原始数据存放在Raw_Data文件夹中，格式为.txt文件。此外，Plotted_Data_Scripts文件夹中包含了由该Excel文件处理得到的所有.txt格式数据，以及用于绘图的Matlab代码。

# 摘要
## 研究假设
我们的假设是：通过分区式EDGE（partitioned-Edge-based Droplet GEneration）张力测量法得到的动态界面张力值，可与经典技术（如自动化滴形张力仪ADT）所得结果相匹配，且能将可测时间尺度拓展至极短区间。
## 实验设计
本研究针对油-水界面与气-水界面展开探究，连续相为乳清分离蛋白（whey protein isolate, WPI）溶液，浓度范围为2.5–10 wt%，分散相为正十六烷纯液或空气。我们采用EDGE张力仪与ADT在不同时间尺度下测量界面（表面）张力，并通过对比分析识别不同蛋白浓度以及油-水与气-水界面之间的差异。
## 研究结果
EDGE张力仪的可测时间尺度低至数毫秒，最高可达约10秒；而ADT仅能在液滴注射至少1秒后获取动态界面张力值，且通常可覆盖至数小时的时间区间。两种技术测得的界面张力值存在重叠区间，表明二者可提供一致且互补的实验数据。与ADT不同，EDGE张力仪能够在油-水与气-水两种界面下，区分最高至10 wt%（本研究测试的最高浓度）的蛋白吸附动力学差异。

# 测量与数据采集
本研究的测量与数据采集流程遵循论文中的描述。我们主要采用两套实验装置与设备开展数据采集，分别为自动化滴形张力仪（Automated Drop Tensiometer, ADT）与分区式EDGE（partitioned-Edge-based Droplet GEneration）。