Improving eDNA detection in sponge natural samplers using blocking primers

OTU Table Metadata File OTU_Table.csv Creator Paul Dreger Date Apr 25 Sequencer MinION Mk1B, R10.4.1. flow cell technology - Oxford Nanopore Technologies Software decona v1.4, R 4.4.2 Variables Barcode: Sample identifier used for tracking samples during metabarcoding uniqueID: Unique sequence ID using Barcode and SeqID SeqID: Identifier of the query sequence used in the BLAST search SseqID: Subject sequence identifier returned by BLAST Total_readcount: Total number of reads associated with the sample/barcode PercIdent: Percentage of identical bases between query and subject sequences Alignmentlength: Length of the alignment between the query and subject sequences Mismatch: Number of mismatched bases in the alignment Gapopen: Number of gap openings in the alignment QueryStart: Starting position of the alignment in the query sequence QueryEnd: Ending position of the alignment in the query sequence SubjectStart: Starting position of the alignment in the reference sequence SubjectEnd: Ending position of the alignment in the reference sequence Evalue: Expectation value; indicates the number of hits expected by chance Bitscore: Score indicating the quality/significance of the alignment Querylength: Total length of the query sequence Subjectlength: Total length of the reference sequence OTU: Operational Taxonomic Unit assigned based on sequence similarity TaxID: Taxon ID from BOLD/NCBI database Read_Count: Number of reads associated with the OUT domain: Domain name of highest ranking BLAST hit phylum: Phylum name of highest ranking BLAST hit class: Class name of highest ranking BLAST hit order: Order name of highest ranking BLAST hit family: Family name of highest ranking BLAST hit genus: Genus name of highest ranking BLAST hit species: Species name of highest ranking BLAST hit warning: Indicates whether sequence has been assigned to do different taxa with the same likelihood Sequence: Full consensus sequence after reclustering Primer: Primer used to inhibit host DNA amplification (G08R/G3F/None) SampleID: Unique sample ID Island: Island on which site is located Site: Location name Sample_or_Control: Label if sample is a true sample or control quant_reading: DNA quantity of PCR product used in sequencing (ng/µl) Abstract This file is the OTU table generated by decona v1.4 from raw FASTQ files (for full command and parameters, see manuscript) containing each consensus sequence with its highest scoring BLAST hit based on E-Value, Percentage Identity and Alignment length. Taxonomic assignment was done using an adjusted version of the "Taxonomy assign after decona.R" script provided on https://github.com/karlijn-doorenspleet/decona-postprocessing. Raw FASTQ files as well as scripts used for assignment and downstream analyses will be made available in online nucleotide databases (which is to be determined) and GitHub, respectively. Key words metabarcoding, otus, blast, taxonomy File type CSV, UTF-8 encoding License CC BY-NC Publication This study