Top gene deletion strategies identified by BiMOMA (k<5) or BiMOMA+Local Search (k>5) for pyruvate production under glucose aerobic condition.

a‘Changes’ refer to the number of genes which are newly introduced in the solution with k deletions or removed from the solution with k–1 deletions. bYield is reported as % of the TMP (2 mol pyruvate produced/mol glucose consumed) for wildtype strain with a maximum glucose uptake rate of 10 mmol gDW−1 h−1 and a maximum oxygen uptake rate of 18.5 mmol gDW−1 h−1.