16S rDNA sequencing of human saliva cultured under anoxic conditions with TMAO and with or without inoculation with Aggregatibacter actinomycetemcomitans

This project includes raw 16S rDNA sequencing data (V4 region) of human saliva cultured under anoxic conditions with TMAO and with or without inoculation with Aggregatibacter actinomycetemcomitans (Aa), a major periodontal pathogen. The goal of the study was to identify taxa that expand in response to trimethylamine N-oxide (TMAO), a common dietary metabolite and preferred anaerobic electron acceptor of Aa. The following conditions were sequenced: (1) Input: Baseline saliva from healthy human donors, before anoxic culture; (2) Control: Saliva supplemented with water (vehicle for TMAO) and incubated under anoxic conditions for 3-4 days; (3) TMAO: Saliva supplemented with 50 mM TMAO and incubated under anoxic conditions for 3-4 days. These three conditions were tested both without Aa inoculation (3-day culture) and with Aa inoculation (4-day culture). Sequencing of the libraries was performed to quantify relative abundance of taxa across conditions.