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PacBio Iso-Seq of pools of eggs and embryos of Atlantic cod

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https://www.ncbi.nlm.nih.gov/sra/ERP020574
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Equal amounts of RNA were isolated from pools of unfertilized eggs and at 20, 30, 45, 60 and 90 days post hatch. Isolations were pooled and three size-selected fractions based on agarose gel-electrophoresis of RNA were created at sizes 1-2 kbp, 2-3 kbp and 3-6 kbp and sequenced on the Pacific Biosciences RS using P6v2-C4 chemistry.
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2021-02-04
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