Expression data from C2C12 myotubes treated with sugarcane top ethanolic extract (STEE) and control C2C12 myotubes.

The aim of the study was to obtain the data on the changes brought by STEE in in vitro myotubes, with a focus on metabolism and mitochondria, to explore its unknown functional properties. In this study, the transcriptome-wide analysis using microarray was performed, whcih provide insights into the biological and molecular changes induced by STEE. The microarray allowed us to capture the transcriptome-wide changes induced by STEE in C2C12 myotubes. For example, STEE induced changes in the expression of genes involved in mitochondria activity, fatty acid metabolism, or inflammatory cytokines in C2C12 myotubes. Microarray analysis was performed in duplicates for each samples; STEE treatment groups were set at two concentrations, 30 µg/mL and 50 µg/mL, and exposed to C2C12 myotubes for 6 hours. RNA extraction from control C2C12 myotubes followed the same schedule as the extraction from STEE-treated C2C12 myotubes.