Differentially expressed miRNAs in mice renal tubular epithelial cells which co-cultured with mesenchymal stem cells vs controls

In order to study the effect of mesenchymal stem cells on miRNAs in renal tubular epithelial cells during renal fibrosis, and to find new treatment methods for renal fibrosis, we used TGF-β1 to stimulate mouse tubular epithelial cells, co-cultured with mesenchymal stem cells for 48 hours, and collected renal tubular epithelial cells .The renal tubular epithelial cells that were only stimulated by TGF-β1 were used as a control group. High-throughput miRNA sequencing was used to detect the increased and decreased miRNAs after co-culture. C57 / BL6 mouse renal tubular epithelial cells were stimulated with TGF-β1 for 48 hours and divided into two groups. One group was used as the experimental group for co-culture with mouse mesenchymal stem cells for 24 hours, and the other group was used as the control group without treatment, each with 3 replicates.