Metadata and datasets supporting the published article: A simple ATAC-seq protocol for population epigenetics

In this study, the authors describe a protocol for the generation of sequence-ready libraries for population epigenomics studies.<br><b>Data access</b>: All the datasets that support the findings of this study are publicly available as supplementary files that accompany the main article. Dataset qPCR cycles.xlsx is available in figshare, as part of this data record (<b>https://doi.org/10.6084/m9.figshare.11394618</b>).<br><br><b>Study aims and methodology: </b>The purpose of this study was to describe a streamlined and robust method for Assay for Transposase Accessible Chromatin (ATAC-Seq) for individuals of the crustacea <i>Daphnia pulex</i> and also for the trematode <i>Schistosoma mansoni</i>.The following procedures are described in more detail in the published article:preparation of animal samples and transposase mixture, chromatin tagmentation, DNA library amplification, animal sampling and transposase mixture, AMPure XP beads double side purification, libraries check. <br><b>Datasets supporting the figures in the published article:</b><b><br></b><b>Data supporting figure 1</b>: Data file <b>qPCR plot 29-05-18.jpg</b> shows an example qPCR amplification profile for four samples.Dataset <b>qPCR cycles.xlsx</b> is an Excel table with the number of library amplification PCR cycles based on qPCR.<br><b>Data supporting figure 2</b>: Data files <b>Agarose picture.Tif</b>, <b>Agarose profile.png</b> and <b>BioAnalyser.pdf </b>show examples of fragmentation profiles. <b>Data supporting figure 3</b>: Data files <b>BioAnalyser 2.pdf</b>, <b>BioAnalyser 3.pdf </b>and <b>BioAnalyser 4.pdf</b> show examples of BioAnalyser profiles of ATAC-seq libraries after fragment size selection.<br><br><br>