Expression of Human Lung Before and After Ex Vivo Lung Perfusion

Ex vivo lung perfusion restores normothermia, ventilation and circulation to donor lungs, typically after a period of cold ischemia. This allows donor lungs to be evaluated prior to transplantation. We used microarrays to study the biological response of human lungs to Ex Vivo Lung Perfusion. Samples were collected from donor lungs at Toronto General Hospital. Lungs were donation after brain death (DBD) Samples were collected at the end of cold ischemia (CIT1). Lungs were then placed on the EVLP circuit which was then followed by a second round of CIT prior to being transplanted. The second time point was collected at the end of the second round of cold ischemia (CIT2).