Effect of SRSF1 depletion on FANCD2 mRNA and isoform expression and effect of FANCD2 depletion or expression of SRSF1 mutants on splicing

To investigate the cooperative function of FANCD2/SRSF1 complex in the regulation of R-loops, we performed gene expression, isoform and splicing analysis in Hela cells depleted of SRSF1 or expressing SRSF1 mutants and FA-D2 mutant (FA-D2) and wild type (FA-D2+FANCD2) cells. FANCD2 gene expression and isoform analysis of RNA-seq data for HeLa cells depleted for SRSF1. Differential splicing analysis of RNA-seq data for HeLa cells expressing SRSF1 mutants or FA-D2 mutant (FA-D2) and wild type (FA-D2+FANCD2) cells.