Data from: Single-cell profiling of muscle-infiltrating T cells in idiopathic inflammatory myopathies

The dataset corresponds to the study by Argyriou, Horuluoglu, Galindo-Feria et. al. Single-cell profiling of muscle-infiltrating T cells in idiopathic inflammatory myopathies. It contains the FASTQ files trimmed by adaptors of RNA-sequencing from single T-cells. There are 3840 cells from muscle biopsies and matched peripheral blood from six donors with idiopathic inflammatory myopathies and one with undefined myopathy. 

The cells were sorted using flow cytometry, and RNA-sequencing libraries were generated with the Smartseq2 at the Eukaryotic Single-cell Genomics facility at Science for Life Laboratory in Stockholm, Sweden. Libraries were sequenced on an Illumina Nova seq 6000 (2*100bp) at NGI Stockholm sequencing core facilities, Science for Life Laboratory in Stockholm, Sweden. Additionally, there are smart-seq-3 single-cell transcriptomics from 439 memory T cells from peripheral blood from three healthy donors.

Terms for access
The dataset is only to be used for medical research seeking to advance the fields of immunology, autoimmune diseases, or genetics. After justification and discussion with the study's authors, other research areas could be considered. 

To access the dataset, investigators must sign a data transfer agreement with the dataset owners and comply with EU General Data Protection Regulation. The investigators granted access to the dataset must commit to only using it for the research described in their application.  The data set should not be shared with other parties. 

For conditional access to the dataset in this publication, please get in touch with [e-mail]

本数据集对应于 Argyriou、Horuluoglu、Galindo-Feria 等人所进行的研究：《特发性炎症性肌病中肌浸润 T 细胞的单细胞分析》。数据集包含来自单个 T 细胞的 RNA 测序的 FASTQ 文件，经过适配体剪切处理。数据集内含有 3840 个来自肌活检和与六位特发性炎症性肌病患者及一位未定义肌病患者的匹配外周血液的细胞样本。细胞通过流式细胞术进行分离，RNA 测序文库则由瑞典斯德哥尔摩科学生命实验室的欧核单细胞基因组学设施使用 Smartseq2 技术构建。文库在斯德哥尔摩生命科学实验室的 NGI 斯德哥尔摩测序核心设施上使用 Illumina Nova seq 6000 测序仪进行测序（2*100bp）。此外，还包括来自三位健康捐献者外周血液中 439 个记忆 T 细胞的 smart-seq-3 单细胞转录组数据。

数据访问条款
本数据集仅限用于旨在推进免疫学、自身免疫性疾病或遗传学领域的医学研究。在经研究作者论证并讨论后，可考虑其他研究领域。

为了访问数据集，研究人员必须与数据集所有者签署数据传输协议，并遵守欧盟通用数据保护条例。获得数据集访问权限的研究人员须承诺仅将其用于申请中描述的研究目的。数据集不得与第三方共享。

关于本出版物中数据集的有限访问条件，请联系 [e-mail]