Single-cell RNA sequencing of small intestine neurons in Hirschsprung disease mice

This study was undertaken to identify gene expression changes in enteric neurons of Hirschsprung disease mice. Methods: We performed single-cell RNA sequencing of enteric nervous system cells isolated from the small intestine of Ednrb-knockout mice at age P14. Results: Through comparisons with published datasets of WT small intestine enteric neurons, we identify a missing neuronal population in the ganglionated small intestine of Hirschsprung disease mice. Conclusion: Single-cell RNA sequencing permits identification of cellular perturbations in Hirschsprung disease models. Use of 10X Genomics technology to analyze single-cell transcriptomics myenteric plexus neurons in an Ednrb-knockout model of Hirschsprung disease.