ISL1-binding sites in human ES cell-derived cardiomyocyte progenitor cells identified by ChIP-seq upon cardiac differentiation

ChIP-Seq analysis of ISL1 binding sites in cardiomyocyte progenitor cells captured at day 5 of directed cardiac differentiation. hES cells (HuES6 background) are deficient in endogenous ISL1 but may express ISL1 under control of a doxycycline-inducible promoter. DOX was added at days 3 and 4 of high-efficiency cardiac differentiation and cells are harvested at day 5, the peak of endogenous ISL1 expression in wild-type cells. Analysed samples comprise a treatment sample expressing ISL1 (DOX added), a minus DOX control without ISL1 expression and an input control. Analysis of ISL1 binding sites in one treatment sample and two different negative controls. Each sample comprises pooled material from several independent experiments.