Investigation of the gene expression in the major surface protein deficient mutant of Treponema denticola

Treponema denticola is involved in chronic periodontitis pathogenesis. The mechanism underlying the regulation of the expression of its virulence factors, such as major surface protein (Msp) is yet to be clarified. We determined the gene expression profiles of Msp-deficient mutants of T. denticola. Gene expression profiles of T. denticola ATCC 35405 (wild type), and msp-deficient mutant, DMSP3, were determined using DNA microarray analysis. In addition to several differentially expressed genes, dentilisin expression was reduced in DMSP3. Potential repressor, TDE_0344, was elevated in DMSP3. Comparison of 2 strains of T. denticola. Treponema denticola ATCC 35405 and DMSP3 were cultured in TYGVS medium for 24-48 h. Profile of gene expression of T. denticola DMSP3 was compared to that of ATCC 35405.