Landscape of CD4+Foxp3+ Treg cells in the liver of neonatal mice

To study the heterogeneity of regulatory T cells (Tregs) in various organs, we performed single-cell RNA sequencing on liver and splenic Treg cells obtained from 12-day and 7-week old mice. Unique Treg subsets in neonatal liver were found and their impact on liver development and maturation was investigated. To fully characterize liver Treg cells in neonatal mice, we performed scRNA-seq on sorted liver and splenic CD4+Foxp3GFP+ Tregs from 12-day-old Foxp3GFP-Cre Rosa26loxP-Stop-loxP-YFP mice. We also compared it with the scRNA-seq of Tregs purified from the liver and spleen of 7-week-old mice.