COS7 pNLGFP INF-alpha

Tilt Series Date: 2012-01-20 Data Taken By: Cora Woodward Species / Specimen: Cercopithecus aethiops (monkey, African green) Strain: COS-7 ATCC CRL-1651 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Acquisition Software: UCSF tomo Upload Method: rundir Processing Software Used: imod Collaborators and Roles: pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected with lipofectamine Sample Preparation: manual blotting