Profiling of melocular mechanism of SOX17 effect during hematopoieisis of human ES cells

Here, using SOX17-knockout and SOX17-inducible human PSCs, paired with cell biology and molecular profiling studies, we revealed that SOX17 represents a critical upstream factor that is required for activation and linkage of HOXA and arterial programs in hemogenic endothelium and establishing definitive lympho-myeloid hematopoiesis. These SOX17 effects are mediated through activation of NOTCH, CDX2 and retinoic acid signaling. Collectively, these findings are important to design strategies for direct HSC fate programming from hPSCs. Overall design: For ATAC-seq, DNA profiles of overexpression of SOX17 and non-overexpressed SOX17 were prepared in duplicate. For ChIP-seq, SOX17 and IgG antibodies in PHE. For RNA-seq, mRNA profiles of overexpression of SOX17 and non-overexpressed SOX17 were generated by sequencing, in triplicate.