EST distributions of carotenogenesis-related genes in the <i>Phaeodactylum</i> Digital Gene Expression Database.

The database was searched for ESTs encoding genes of the diatom carotenoid biosynthetic pathway (see text for abbreviations of the genes) and their distribution in the different libraries was determined. The numbers given in the table reflect the amount of ESTs per one thousand ESTs (‰) for each respective library. Also given are the EST contig ID numbers from the Gene Expression Database v3.0 and the total number of ESTs in each library. Abbreviations used for the libraries: OS) Original 12000 Standard, OM) Oval Morphotype in 10% seawater, TM) Triradiate Morphotype, TA) Tropical Accession at 15C°, SM) Silica Minus in artifical seawater, SP) Silica Plus in artificial seawater with 350 uM metasilicate, NR) Nitrate Replete with 1.12 mM nitrate in chemostat, NS) Nitrate Starved for 3 days with 50 µM nitrate in chemostat, AA) Ammonium Adapted at 75 µM ammonium, UA) Urea Adapted at 50 µM urea, FS) Iron Starved at 5 nM iron, BL) Blue Light for 1 h on 48 h dark adapted cells, LD) Low Decadienal Treated for 6 h with 0.5 ug/ml 2E,4E-decadienal, HD) High Decadienal Treated for 6 h with 5 ug/ml 2E,4E-decadienal, C1) 230 µM/Kg of CO2for 1 day in chemostat, C4) 230 µM/Kg of CO2 for 4 days in chemostat. Note: this table gives LTL11 and LTL2 as alternative genes involved in β-carotene hydroxylation, because BCH is absent in P. tricornutum.