RiboTag and bulk RNA sequencing using AAV-shGpr151 and AAV-shGpr151 with AAV-e5'UTR infected sensory neurons.

After discovering that Gpr151 mRNA regulates the CSDE1-mediated rp-mRNA operon, we designed an engineered version of the 5'UTR of Gpr151 mRNA(e5'UTR), which has an enhanced interaction with CSDE1. To determine if this e5'UTR RNA can restore the down-regulated rp-mRNA operon formation in Gpr151 knockdowned neurons, we performed total RNA and RiboTag sequencing analysis using AAV-shGpr151 or AAV-shGpr151 with AAV-e5'UTR infected sensory neurons using RiboTag mice, RPL22HA+/+;Advillin-Cre+. Overall design: RiboTag sequencing data from cultured sensory neurons infected with AAV-shGpr151 and AAV-shGpr151 with AAV-e5'UTR. Preconditioning through sciatic nerve injury was conducted on mice before the neuron culture to activate their regenerative state.