Host/microbial cross-talk in the gut: In-depth investigation of the symbiotic effects of Roseburia hominis

Here, we report analysis of both the bacterial and host transcriptome as affected by colonization of R. hominis in the mouse gut. Microbial genes required for colonization and adaptation in the murine gut, as well as host genes responding to colonization by this bacterial species, were uncovered. Germ-free (GF) male mice were gavaged at 0d with 100 uL of R. hominis culture. Mice were sacrificed on 14d and 28d post-colonization in parallel to age-matched GF controls.