Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex

DNA damage induced activation of the checkpoint kinases Chk1/Chk2(Cds1) results in the conversion and/or maintenance of CyclinB:Cdc2 complex in its Tyrosine 15 phosphorylated (inactive) state. Cdc2 activity is regulated by a balance between the phosphorylation and dephosphorylation by the Wee1/Myt1 kinase and Cdc25 phosphatase. Inactivation of the Cyclin B:Cdc2 complex likely involves both inactivation of Cdc25 and/or stimulation of Wee1/Myt1 kinase activity.

DNA损伤触发的关卡激酶Chk1/Chk2（Cds1）的激活，导致CyclinB:Cdc2复合物在酪氨酸15位磷酸化（非活性）状态下的转化与/或维持。Cdc2的活性通过Wee1/Myt1激酶的磷酸化与Cdc25磷酸酶的去磷酸化之间的平衡进行调控。CyclinB:Cdc2复合物的失活可能涉及Cdc25的失活以及/或Wee1/Myt1激酶活性的刺激。