The Scc2NIPBL/Scc4MAU2 complex acts in sister chromatid cohesion and transcriptional regulation by maintaining nucleosome-free regions [microarray]
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE56991
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The Scc2/Scc4 complex binds to broad nucleosome-free regions in the promoters of highly expressed genes. The cohesin loader is recruited to these sites by the RSC chromatin remodeling complex Total RNA was isolated from wt, scc2-4 and sth1-3 cells at 25°C and 37°C. dscDNA was obtained using the IVT system (Invitrogen) and hybridized to Affymetrix Yeast Genome 2.0 Arrays to analyze the expression profile of wt, scc2-4 and sth1-3 cells.
创建时间:
2017-02-21



