In-Depth Proteome Coverage of In Vitro-Cultured Treponema pallidum and Quantitative Comparison Analyses with In Vivo-Grown Treponemes

Previous mass spectrometry
(MS)-based global proteomics studies
have detected a combined total of 86% of all Treponema
pallidum proteins under infection conditions (in vivo-grown T. pallidum). Recently, a method was developed for
the long-term culture of T. pallidum under in vitro conditions (in vitro-cultured T. pallidum). Herein, we used our previously reported optimized MS-based proteomics
approach to characterize the T. pallidum global protein expression profile under in vitro culture conditions.
These analyses provided a proteome coverage of 94%, which extends
the combined T. pallidum proteome coverage
from the previously reported 86% to a new combined total of 95%. This
study provides a more complete understanding of the protein repertoire
of T. pallidum. Further, comparison
of the in vitro-expressed proteome with the previously determined
in vivo-expressed proteome identifies only a few proteomic changes
between the two growth conditions, reinforcing the suitability of
in vitro-cultured T. pallidum as an
alternative to rabbit-based treponemal growth. The MS proteomics data
have been deposited in the MassIVE repository with the data set identifier
MSV000093603 (ProteomeXchange identifier PXD047625).

既往基于质谱技术（MS）的全球蛋白质组学研究已检测到在感染条件下（体外培养的苍白密螺旋体，T. pallidum）全部苍白密螺旋体蛋白的86%。近期，一种在体外条件下长期培养苍白密螺旋体的方法得到开发（体外培养的苍白密螺旋体）。本研究中，我们利用先前报道的基于MS的蛋白质组学优化方法，对体外培养条件下苍白密螺旋体的全局蛋白表达谱进行了表征。这些分析实现了94%的蛋白质组覆盖度，将先前报道的苍白密螺旋体蛋白质组覆盖度86%提升至新的综合总覆盖度95%。本研究提供了对苍白密螺旋体蛋白库的更全面理解。此外，将体外表达的蛋白质组与先前确定的体内表达的蛋白质组进行比较，仅在两种生长条件之间发现了少数蛋白质组变化，进一步证实了体外培养的苍白密螺旋体作为基于家兔的密螺旋体生长的替代品的适宜性。MS蛋白质组学数据已存入MassIVE数据存储库，数据集标识符为MSV000093603（蛋白质组交换标识符PXD047625）。