Evaluating the Benefits and Limits of Multiple Displacement Amplification with Whole-Genome Oxford Nanopore Sequencing

The objective of this project is to assess the impact of multiple displacement amplification on long-read sequencing across various species, including gram-positive and gram-negative bacteria, as well as protozoan parasites. We assess the impact using low-concentrations, DNA integrity impact, and amplification impact to generate contiguous genome assemblies. The goal of this approach is to use this method as a solution to get contiguous genomes from rare samples.