Further inhibition of Histone H2A ubiquitination protects myocardium against ischemia/reperfusion injury through enhancing glycolysis and improving mitochondrial function via promoting Hsp27 expression [I]

RNA-seq was undertaken in rats with or without myocardial ischemia-reperfusion injury to derive a list of signaling pathways and differentially expressed genes Ischemia was observed when the left anterior descending (LAD) coronary artery was ligated around the PE-10 tube with moving nodule with 6-0 sutures, and occlusion of the left anterior descending coronary artery was confirmed by elevation of the ST segment of the electrocardiogram. Ischemia is released after 30 min for reperfusion. 6 h after reperfusion, the heart was harvested for the following experiment.