Multiple myeloma and health individuals exosomal miRNAs expression profiling

Multiple myeloma (MM) is a hematopoietic malignancy. Based on its laboratory and clinical presentation, it can be categorized as MGUS, smoldering myeloma, and multiple myeloma. Previous studies have indicated that the expression levels of miRNAs differ across various stages of the disease, and exosomes are involved in modulating the progression and metastasis of cancers through miRNAs. This study aims to analyze the expression levels of miRNAs in the serum exosomes of healthy individuals and those with myeloma bone marrow. Overall design: BM samples were obtained from 7 MM patients and 3 healthy individuals .The exosomes from bone marrow were isolated by ultracentrifugation. Exosomal miRNA profiling was sequencing on Illumina NextSeq 500.