Analysis of CTCF-mediated chromatin interactions at the BCL11A locus in HUDEP-2 cells by Micro-Capture-C (MCC)

Three-dimensional chromatin structures, including promoter-enhancer interactions and CTCF looping, are essential for gene regulation. In this study, we apply Micro-Capture-C (MCC) to analyze chromatin structures at the BCL11A locus. Overall design: Micro-Capture-C was performed at the BCL11A locus using probes targeting seven CTCF binding sites (CBS) as baits, with experiments conducted in two biological replicates.