Single-cell RNA sequencing reveals the heterogeneity of liver-resident immune cells in human.

The liver plays a critical role in both immune defense and tolerance in the body. The liver-resident immune cells (LrICs) determine the immune properties, but the unique composition and heterogeneity of these cells are incompletely understood. Here, we dissect the diversity of LrICs by a comprehensive transcriptomic profiling using unbiased single-cell RNA-sequencing (scRNA-seq). Overall design: 10x genomics was used to measure CD45+ immune cells in healthy human liver and paired spleen and PBMCs from the same donors.