RNA sequencing of Sphk1fl/fl Sphk2-/- and Sphk1-/- Sphk2-/- mESC clones.
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE139964
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资源简介：
We report a growth defect in sphingosine kinase null clones due to a cell cycle arrest in G2/M phase resulting in a growth defect. GSEA showed upregulation of the G2M checkpoint geneset. Further, telomere elongation factors are upregulated in kinase null cells. mESC clones derived from Sphk1fl/fl Sphk2-/- mice were cultured in tamoxifen for 48 hours then sorted for Cre-GFP expression yielding GFP- Sphk1fl/fl Sphk2-/- cells and GFP+ Sphk1-/- Sphk2-/- (Kinase Null) cells. This was done for 2 separate clones resulting in 4 sample types: Clone 4 GFP-, Clone 4 GFP+, Clone 10 GFP- and Clone 10 GFP+.
创建时间：
2020-02-05
