Expression data from Hela cells and HSF2-deficient HeLa cells subjected to heat treatment in the presence of IHSF115, an inhibitor of HSF1.. Expression data from Hela cells and HSF2-deficient HeLa cells subjected to heat treatment in the presence of IHSF115, an inhibitor of HSF1.

The majority of heat-induced genes were inhibited by IHSF115, i.e. were positively regulated by HSF1, suggesting that HSF1 plays a predominant role in the transcription of heat-induced genes IHSF115 effectively countermanded repression in a significant fraction of heat-repressed genes, suggesting that repression of these genes is mediated by transcriptionally active HSF1. Overall design: Transcriptomic changes in cells incubated for 2h at 37ºC with vehicle (DMSO) or IHSF115, subjected to heat treatment at 43ºC for 30min in a thermostatically controlled water bath and then incubated for an additional 1h at 37ºC. The assays were conducted by triplicate (HeLa cells) or quintuplicate (HSF2-deficient Hela cells).