Quantitative analysis of transcriptomes in irradiated/unirradiated pancreatic cancer cells

Irradiated dying tumor cells play crucial roles in tumor repopulation. However, the transcriptome changes in pancreatic cancer cells after radiation remains unknown. Here we performed RNA-sequencing in pancreatic cancer cell line SW1990 and PANC-1 that were 10Gy irradiated or unirradiated. Bioinformatic analysis revealed that the transcriptomes were significantly changed after radiation. Gene Ontology (GO) analysis revealed that the signaling concerning extracellular vesicles, especially exosomes, were significantly enriched. Further investigation revealed the vital role of irradiated dying tumor cells-derived exosomes in potentiating tumor repopulation. Overall design: Examination of transcriptome changes after radiation in 2 cell types.