Gene expression profiles proton-induced in brest cell lines

Literature data report the helpful role of specific molecularly based signatures to predict response to treatment in cancer, including breast cancer (BC) disease. As known, BC is a heterogeneous disease presenting distinct subtypes with different clinical outcomes. Thus, the choice of a unique treatment plan for all BC patients, including radiation therapy (RT), may be not the best option. Technological advances in RT are evolving with the use of proton beams, which reduce the dose administered to the heart compared to conventional RT. However, limitate data regarding proton-induced molecular changes are currently available. The aim of this study was therefore to study gene expression profiles induced by proton irradiation with 0.5, 2 and 9 Gy of Ionizing radiation (IR) doses in breast cells. The great amount of data here collected, represent an useful tool to better understand the molecular mechanisms elicited by proton irradiation, thereby filling the existing lack of data in the literature. Proton-induced gene expression changes in non- tumorigenic (MCF10A) and in tumorigenic breast (MCF7 and MDA-MB-231) immortalized cell lines were analyzed as two-color hybridizations using Agilent Technologies whole human genome 4x44K microarrays. New Samples were named as follow: MCF10A_0,5Gy_proton; MCF10A_2Gy_proton; MCF7_0,5Gy_proton; MCF7_2Gy_proton; MDA-MB-231_0,5Gy_proton; MDA-MB-231_2Gy_proton. In addition, we have re-used the previous samples in GSE103472 (18 samples out of 37 as included in the current records).