The paired architecture of Rad51-Dmc1 co-foci is independent of strand exchange and synapsis.

(A,B) Micrographs of (A) mnd1 and (B) zip1 mutant nuclei in the spo11 hypomorphic tetraploid background. Scale bar = 1 μm, 400 nm for the inset. Rad51 staining is shown in green, Dmc1 in red, and DAPI in blue. (C-F) Accompanying distribution of nearest neighbor measurements as in Fig 1 for (C) Rad51→Rad51, (D) Dmc1→Dmc1, (E) Rad51→Dmc1, and (F) Dmc1→Rad51. Micrographs from cultures 2.5 hours after meiotic induction. Wild type data repeated from Fig 1 for comparison. Scoring of 70 and 44 focus-positive nuclei; 6,364 and 2,824 Rad51 foci; and 6,114 and 2,655 Dmc1 foci are included in histograms for mnd1 and zip1 mutants, respectively.