Immunocytochemistry Protocols.
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Microwave protocol: 18°C; Power level 2; 2 min on/2 min off/2 min on/2 min off.Pella research-grade oven (# 3450, with variable power controller #3430 and cold spot).A: sodium azide.Alexa 564-Donkey anti-goat tertiary (Molecular Probes #A11056).BSA: bovine serum albumin, IgG-free (Jackson Immunoresearch #001-000-161).Cy3-Goat anti-mouse secondary (Jackson #115-165-068).Cy3-Goat anti-rabbit secondary (Jackson # 111-165-144).Cy3-Goat anti-rabbit F(ab)2 (Jackson # 111-166-047).DNAse I (Sigma): 10 U DNAse, 4 mM MgCl2 in PBS; 1 hr, 37°C.G: glutaraldehyde.M/F: methanol/37% formalin.NaBH4: 0.01 M NaBH4, 0.5% SMB in 0.05 M Tris HCl, pH 7.5; 30 min.NGS: normal goal serum.ON: overnight on a shaker.P: paraformaldehyde.PB: phospate buffer pH 7.4.PBS: phosphate-buffered saline (10 mM sodium phosphate, 150 mM NaCl, pH 7.4).RT: room temperature.SMB: sodium metabisulfite.T: Triton X-100.TB: 10 mM Tris-HCl, pH 7.4.TBS: Tris-buffered saline (20 mM Tris-HCl, 150 mM NaCl, pH 7.4).
创建时间：
2015-12-02
