Human HEK293 Gemin5 phosphorylation site T897 mutants LC-MSMS

Identification of cellular proteins interacting with Gemin5 phosphorylation mutants in T897 residue. We used the T897A mutant as phosphorylation defective protein and the T897E mutant as phosphomimetic. P85 C-terminal fragment of Gemin5 has been used to purify the protein complexes associated by Tandem Affinity Purification. The study has been performed using two biological replicates of each bait protein: p85-T897A and p85-T897E.