RNA-seq analysis upon ARID1B overexpression

We transduced either an empty vector or ARID1B cDNA in IMR90 cells. Cells were selected with puromycin and 6 days after the infection we collected the RNA. ARID1B is a member of the SWI/SNF chromatin-remodeling complex. Our previous experiments showed that knockdown of ARID1B allows cells to bypass cellular senescence. By performing RNA-seq we have shown that ARID1B expression can induce a set of genes involved in the senescence response.我们在IMR90细胞中转导空载体（empty vector）或ARID1B互补DNA (cDNA)。随后使用嘌呤霉素（puromycin）筛选细胞，并于转导后第6天收集并提取RNA。ARID1B是SWI/SNF染色质重塑复合物（SWI/SNF chromatin-remodeling complex）的成员之一。我们此前的实验结果显示，敲低ARID1B可使细胞绕过细胞衰老（cellular senescence）进程。通过RNA测序（RNA-seq）实验，我们证实ARID1B的表达能够诱导一批参与衰老应答的基因的表达。