HERVK HML-2 Target Enrichment pilot experiment

DNA was extracted from control human genomic DNA. Genomic regions of interest were selected using a biotin-streptavidine based bead capture with DNA bait probes. In this case, target-specific baits used came from the beginning and end of HK2 LTR – approximately 360 bp from each end. Five overlapping probes were used for each end; each probe was 120 bp in length. After target enrichment hybridization, the sample was sequenced using the Illumina MiSeq platform producing PE 300 bp paired-end reads.