Effect of rice bran fermented with Saccharomyces cerevisiae Misaki-1 and Lactobacillus plantarum Sanriku-SU8 on gut microbiome of mice fed short-term with high-sucrose and low-dietary fibre diet. mouse gut metagenome

To clarify the effect of rice bran (RB) and fermented RB (FRB) in a high-sucrose and low-dietary fibre diet on the gut microbiome, the in vitro bile acid-lowering capacity and caecal microbiota of ICR mice fed 20% RB or FRB diets for two weeks were determined. The caecal microbiome was analysed by 16S rDNA gene amplicon sequencing. The in vitro bile acid-lowering capacity was high for FRB. In mouse experiments, triacylgricerol and total cholesterol were generally lower with FRB, although the faecal frequency was highest in mice fed RB. At the phylum level in the caecal microbiome, Firmicutes and Bacteroidetes were high with FRB and RB, respectively. At the OTU level, some bacterial groups related to diabetes and gut toxicity, such as Lachnospiraceae and Enterorhabdus mucosicola, were high for RB but not for FRB. Based on these results, FRB, rather than RB intake, improved the intestinal environment and metabolic activity.