The DNA methylation profiles of liver tissues (normal, non-tumor and tumor tisuues) of F2 males of C3H mice born to F1 mice gestationally exposed to arsenite

Background: Previously, we showed that gestational arsenite (As) exposure of F0 females increases hepatic tumors in the F2 males in C3H mice. However, the mechanisms have not yet been clarified. Methods: We detected genome wide site-specific DNA methylation by using reduced representative bisulfite sequence (RRBS) method. We used the data uploaded here and those with GSE Series accession number GSE111420 (CCN and CCTt) to detect differential methyrated cytosines between control groups and arsenite groups. Results: We clarified that DNA methylation of Tmem54 and Cd74 around TSS were downregulated and their gene expressions were accordingly upregulated in the liver tumor tissues of F2 males of arsenite group. Conclusions: Our study proposes novel DNA methylation dependent pathways which increase liver tumors in the F2 males of arsenite group. Examination of liver DNA methylation modifications (normal tissues (N), non-tumor tissues (Tn), tumor tissues (Tt) of control (CC) and arsenite (AA) group, respectively (N=3/group, total 18 samples)) . These methylation status in CCN and CCTt (N=3) were already publicly avairable at Gene Expression Omnibus (GEO), accession number GSE111420. We uploaded the rest liver DNA methylation status in CCTn, AAN, AATn and AATt (N = 3).