Table_1_New Insights Into the Skin Microbial Communities and Skin Aging.DOCX

Although it is well-known that human skin aging is accompanied by an alteration in the skin microbiota, we know little about how the composition of these changes during the course of aging and the effects of age-related skin microbes on aging. Using 16S ribosomal DNA and internal transcribed spacer ribosomal DNA sequencing to profile the microbiomes of 160 skin samples from two anatomical sites, the cheek and the abdomen, on 80 individuals of varying ages, we developed age-related microbiota profiles for both intrinsic skin aging and photoaging to provide an improved understanding of the age-dependent variation in skin microbial composition. According to the landscape, the microbial composition in the Children group was significantly different from that in the other age groups. Further correlation analysis with clinical parameters and functional prediction in each group revealed that high enrichment of nine microbial communities (i.e., Cyanobacteria, Staphylococcus, Cutibacterium, Lactobacillus, Corynebacterium, Streptococcus, Neisseria, Candida, and Malassezia) and 18 pathways (such as biosynthesis of antibiotics) potentially affected skin aging, implying that skin microbiomes may perform key functions in skin aging by regulating the immune response, resistance to ultraviolet light, and biosynthesis and metabolism of age-related substances. Our work re-establishes that skin microbiomes play an important regulatory role in the aging process and opens a new approach for targeted microbial therapy for skin aging.

尽管众所周知，人类皮肤老化伴随着皮肤微生物群的改变，但对于这些变化在老化过程中的组成及其对老化相关皮肤微生物的影响，我们知之甚少。通过16S核糖体DNA和内部转录间隔区核糖体DNA测序技术，对80名不同年龄段个体（年龄范围不等）的160份皮肤样本（采集于面颊和腹部两个解剖部位）进行微生物群分析，我们建立了与内在皮肤老化和光老化相关的微生物群特征，以深化对皮肤微生物组成随年龄变化依赖性的理解。根据研究结果，儿童组的微生物组成与其它年龄组存在显著差异。进一步的临床参数和功能预测分析揭示了九种微生物群落（即蓝藻、葡萄球菌、表皮葡萄球菌、乳酸杆菌、棒杆菌、链球菌、奈瑟菌、念珠菌和马拉色菌）和18条代谢途径（如抗生素的生物合成）的高丰度，可能影响皮肤老化，暗示皮肤微生物群可能通过调节免疫反应、紫外线抵抗以及老化相关物质的生物合成和代谢等关键功能，在皮肤老化过程中发挥重要的调节作用。我们的研究重新确立了皮肤微生物群在老化过程中的重要调节作用，并为针对皮肤老化的靶向微生物治疗提供了新的途径。