Confirmed and predicted [Fe-S] cluster-containing proteins in Plasmodium.

aGene IDs of the P. berghei and P. falciparum orthologs and the predicted localizations of the proteins were retrieved from PlasmoDB (http://PlasmoDB.org) or identified by similarity searches using A. thaliana [Fe-S] cluster proteins as query sequences [41].bPutative targeting of the P. falciparum [Fe-S] cluster-containing proteins to the apicoplast or mitochondrion was predicted using four different algorithms. PlasmoAP [51] indicates the likelihood of the presence of the required signal peptide followed by the likelihood of an apicoplast localization (“−” = unlikely, “0″ = undecided, “+” = likely, “++” = very likely). ApicoAP [52] is a different algorithm that can identify apicoplast proteins in multiple Apicomplexa (“No SP” = no signal peptide, “No ATP” = signal peptide but no transit peptide, “ATP” = apicoplast targeted protein). PlasMit [53] predicts the likelihood of a mitochondrial localization for P. falciparum proteins (“non-mito” (99%), “possibly” (91%), and “mito” (99%)). MitoProtII [54] gives a probability score for the likelihood of mitochondrial localization but is not optimized for Plasmodium sequences.