Integration assay expressing trwC+/−trwA in the recipient cell.

Donor cells harbouring the suicide mobilizable plasmid pR6K::oriTP oriTw were either S17.1 λpir for TraI-mediated mobilization, or II1 (pCIG1077) for TrwC-mediated mobilization. Recipient strain was DH5α containing the indicated recipient and helper plasmids. The promoter driving the expression of trwC or trwA+trwC is indicated. The inducible Ptac promoter was assayed with (+IPTG) or without (−IPTG) 0,5 mM IPTG in the mating plate. Data are the mean of at least three independent assays. NT, not tested.