Gating of single recombinant human CaV1.2 in HEK293 cells is differentially modulated by several coexpressed human cardiac auxiliary β-subunits

Single-channel gating parameters of human cardiac CaV1.2 stably expressed in HEK293 cells transiently cotransfected with several auxiliary β-subunits and a human cardiac α2δ-2-subunit. Coexpression with β2a and β2b reveals strongest stimulation of single channels compared to control cells without transfection of any β-subunit (ctr). Data were obtained by patch-clamp recordings using cell-attached configuration (charge carrier: 110mM Ba2+; holding potential: −100 mV; test potential: +10 mV for 150 ms). *p(*)p = 0.07 in Bonferroni-corrected post-hoc tests versus control, following one-way ANOVA. Numbers of experiments given in parentheses indicate number of experiments with only one channel in the patch.