LMAS Test Dataset - NIBSC Gut DNA Reference

The twenty bacterial replicons of the National Institute for Biological Standards and Control (NIBSC) Gut Community Standards were used as reference. It includes the following strains: Species Culture collection number Accession numbers Status Gut-Mix-RR Coverage (x) Gut-HiLo-RR Coverage (x) Akkermansia muciniphila DSM 22959 NC_010655 Complete genome 33.33 0.94 Alistipes finegoldii DSM 17242 NC_018011 Complete genome 16.94 4.85 Anaerostipes hadrus DSM 3319 NZ_KB290627 Complete genome 24.04 6.89 Bacteroides thetaiotaomicron DSM 2079 NC_004663 Complete genome 5.99 17.19 Bacteroides uniformis DSM 6597 GCF_000154205 Scaffold 10.81 3.10 Bifidobacterium longum subsp. infantis DSM 20088 NC_011593 Complete genome 29.52 84.63 Bifidobacterium longum subsp. longum DSM 20219 GCF_900104835 Contig 40.19 115.10 Blautia wexlerae DSM 19850 GCF_000484655 Scaffold 11.68 0.34 Clostridium butyricum DSM 10702 GCF_000409755 Contig 11.21 32.09 Collinsella aerofaciens DSM 13712 GCF_902501475 Contig 44.03 12.61 Escherichia coli DSM 1103 CP009072 Complete genome 8.86 25.34 Eubacterium hallii DSM 3353 GCF_000173975 Contig 21.79 6.25 Faecalibacterium prausnitzii DSM 17677 NZ_CP048437 Complete genome 24.66 0.72 Lactobacillus gasseri DSM 20077 NC_008530 Complete genome 66.00 1.91 Parabacteroides distasonis DSM 20701 NC_009615 Complete genome 10.20 29.26 Prevotella copri DSM 18205 GCF_000157935 Scaffold 19.23 55.11 Prevotella melaninogenica DSM 7089 NC_014370,1 and NC_014371,1 2 Chromosomes 23.49 6.73 Roseburia hominis DSM 16839 NC_015977 Complete genome 18.31 5.24 Roseburia intestinalis DSM 14610 NZ_LR027880 Complete genome 12.04 0.34 Ruminococcus gauvreauii DSM 19829 GCF_000425525 Scaffold 14.04 0.41 The raw sequence data of the mock communities, with an even and staggered distribution of species, is available at: SRR11487941 - Gut-Mix-RR Illumina MiSeq sample SRR11487935 - Gut-Mix-HiLo Illumina MiSeq sample